Journal: Molecules (Basel, Switzerland)

Article Title: Effect-Directed Profiling of Akebia quinata and Clitoria ternatea via High-Performance Thin-Layer Chromatography, Planar Assays and High-Resolution Mass Spectrometry.

doi: 10.3390/molecules28072893

Figure Lengend Snippet: Figure 1. HPTLC profiles of A. quinata extracts (A1−A3 leaf and A4 fruit, 100 or 700 µg/band) at UV/Vis/FLD, and after derivatization with the p-anisaldehyde sulphuric acid reagent, and after several planar assays (A. fischeri bioluminescence depicted as greyscale image), separated on HPTLC plates silica gel 60 F254 (without F254 for the SOS-Umu-C bioassay) with ethyl acetate–methanol– water–acetic acid (70:15:15:1, V/V/V/V).

Article Snippet: Aliivibrio fischeri bacteria (NRRI–B11177, strain 7151) and Bacillus subtilis bacteria (DSM-618) were purchased from Leibniz Institute, DSMZ, German Collection of Microorganisms and Cells Cultures (Berlin, Germany).

Techniques: High Performance Thin Layer Chromatography, Bioassay

Journal: Molecules (Basel, Switzerland)

Article Title: Effect-Directed Profiling of Akebia quinata and Clitoria ternatea via High-Performance Thin-Layer Chromatography, Planar Assays and High-Resolution Mass Spectrometry.

doi: 10.3390/molecules28072893

Figure Lengend Snippet: Figure 2. HPTLC profiles of C. ternatea flower extracts (C1−C4, 100−700 µg/band) at UV/Vis/FLD, after derivatization with the p-anisaldehyde sulphuric acid reagent and after several planar assays (A. fischeri bioluminescence depicted as greyscale image), separated on HPTLC plates silica gel 60 F254 (without F254 for the SOS-Umu-C bioassay) with ethyl acetate–methanol–water–acetic acid (70:15:15:1, V/V/V/V).

Article Snippet: Aliivibrio fischeri bacteria (NRRI–B11177, strain 7151) and Bacillus subtilis bacteria (DSM-618) were purchased from Leibniz Institute, DSMZ, German Collection of Microorganisms and Cells Cultures (Berlin, Germany).

Techniques: High Performance Thin Layer Chromatography, Bioassay

Journal: Cell

Article Title: A Dual-Mechanism Antibiotic Kills Gram-Negative Bacteria and Avoids Drug Resistance

doi: 10.1016/j.cell.2020.05.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains E. coli lptD4213 ΔthyA This paper ZG1599 B. subtilis 168 CRISPRi Library Jason Peters and Carol Gross Peters et al., 2016 Detailed bacterial strain information, including strain identifiers, growth media and temperature, is provided in Table S1 N/A N/A Chemicals, Peptides, and Recombinant Proteins SCH-7979 Tocris Biosciences 1592 Ampicillin MP Biomedicals 190148 Cumene Sigma PHR1210 Daptomycin TCI D4229 Gentamicin sulfate Sigma G1914 Meropenem trihydrate TCI M2279 Nisin MP Biomedicals 155839 Novobiocin sodium salt MP Biomedicals 0215595705 Polymyxin B sulfate salt Sigma P1004 Purified E. coli dihydrofolate reductase (FolA) Genscript N/A DAPI ThermoFisher D1306 SYTOX Green Dead Cell Stain ThermoFisher S34860 FM4–64 Dye ThermoFisher T13320 Critical Commercial Assays BacLight Bacterial Membrane Potential kit ThermoFisher B34950 Colorimetric Dihydrofolate Reductase Assay Kit Sigma CS0340 Deposited Data Proteomics ProteomeXchange Consortium PXD013673 Flow cytometry FlowRespository FR-FCM-Z2JD Raw images and Metabolomics Data This Paper; DataSpace at Princeton University N/A Experimental Models: Cell Lines HEK293 ATCC CRL-1573 HK2 ATCC CRL-2190 HLF Cell Applications 506K-05a PBMC TPCS PB010C Experimental Models: Organisms/Strains Male CD-1 mice Pharmaron N/A Female ovariectomized BALB/c mice Pharmacology Discovery Services Taiwan N/A Galleria mellonella PetCo 2336624 Software and Algorithms MATLAB MathWorks https://www.mathworks.com/products/matlab.html Fiji Eliceiri/LOCI group at University of Washington https://imagej.net/Fiji R Studio R Studio https://rstudio.com/products/rstudio/ FlowJo FlowJo, LLC https://www.flowjo.com/solutions/flowjo/downloads Other Synergy HT microplate reader BioTek N/A InfiniteM200 Pro microplate reader Tecan N/A 27G × 0.5 inch needle BD Biosciences 305109 QuantaMaster 40 Spectrophotometer HORIBA Instruments N/A Open in a separate window KEY RESOURCES TABLE SCH-79797 kills Gram-negative and Gram-positive bacteria with undetectable resistance It works by simultaneously targeting folate metabolism and membrane integrity SCH’s dual-targeting is synergistic, but only when on the same chemical scaffold Irresistin-16, an SCH derivative, effectively treats mouse N. gonorrhoeae infection

Techniques: Virus, Recombinant, Purification, Staining, Membrane, Reductase Assay, Flow Cytometry, Software, Spectrophotometry